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Fig. 2 | BMC Veterinary Research

Fig. 2

From: In situ detection of GM1 and GM2 gangliosides using immunohistochemical and immunofluorescent techniques for auxiliary diagnosis of canine and feline gangliosidoses

Fig. 2

Immunohistochemical findings for the detection of GM1 ganglioside in animals affected and unaffected with gangliosidoses. The immunohistochemical technique for the detection of GM1 ganglioside was performed on paraffin-embedded sections of the cerebral cortex from the following animals: a dog (a) and a cat (b) affected with GM1 gangliosidosis; a dog (c) and a cat (d) affected with GM2 gangliosidosis; an unaffected control dog (e) and cat (f). For the detection of GM1 ganglioside, biotinylated cholera toxin B subunit and peroxidase-labeled streptavidin were used. The immunoreactivity was detected by 3,3′-diaminobenzidine as a peroxidase substrate. The sections were counterstained with hematoxylin. Bar = 50 μm

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BMC Veterinary Research

ISSN: 1746-6148

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