Fig. 5From: Construction of a camelid VHH yeast two-hybrid library and the selection of VHH against haemagglutinin-neuraminidase protein of the Newcastle disease virusDetection of interaction between VHH and NDV virion by Dot blot. Purified NDV (a-p) or allantoic fluid (n1 and n2) were spotted on a PVDF membrane for blot. VHHs binding were visualized using HRP labeled anti-His Mabs While positive serum was visualized using HRP labeled anti-chicken antibody. a-g represent VHH1-VHH7; p represent positive serum; n1 represent VHHs (VHH 1 to VHH 7); n2 represent positive serumBack to article page