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Fig. 4 | BMC Veterinary Research

Fig. 4

From: NLRP3 inflammasome activation contributes to Listeria monocytogenes-induced animal pregnancy failure

Fig. 4

LM activates NLRP3 inflammasome by LLO in mouse B6 macrophages (a) IL-1β levels in the culture medium of mouse B6 cells and its derived gene knock-out cells infected with wild-type or Δhly LM was measured by ELISA. b IL-1β levels in the culture medium of wild-type and NLRP3−/− B6 cells infected with wild-type LM and Δhly LM, respectively. c and (d) Western blot for detection of activated caspase-1 (P10) in wild-type and NLRP3−/− B6 cells infected with LM or Δhly LM. LPS + ATP as a positive control of NLRP3 inflammasome activation. LPS + dA:dT (1.5 μg/106 cells) as a positive control of AIM2 inflammasome activation. The data in (a) and (b) are shown as means ± standard deviation from three independent experiments. * p < 0.05; ** p < 0.01. NS: no significance

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