Fig. 4

NEFA effects on nitric oxide production in BUVECs. a Basal DAF fluorescence (−), and after treatment with 0.1 % ethanol, 300 μM myristic acid (MA), 300 μM stearic acid (SA), 0.1 % DMSO (D), 300 μM palmitic acid (PA), 300 μM oleic acid (OA), or 300 μM linoleic acid (LA) for 5 min at 37 °C. b DAF fluorescent signal after incubation with 300 μM LA for 5 min, normalized to the signal without fatty acids. The cells were incubated with BAPTA, EGTA, or BAPTA + EGTA before treatment with LA. c Effect of L-NAME and GW1100 on the LA-induced increase in the DAF signal. Basal fluorescence. DAF fluorescence is expressed in arbitrary units (a.u.). Each bar represents the mean ± SEM of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.005