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Table 2 Summary of the scrapie transmission results in sheep transfused with PBMC, monocytes or T lymphocytes from preclinical sheep with classical ovine scrapie infection

From: Classical scrapie prions are associated with peripheral blood monocytes and T-lymphocytes from naturally infected sheep

Group

Donor ID

Cell type

Cell numbera

Lamb PRNP

 

PrPSc detection by IHCb

sPMCA

   

Antemortem

Postmortem

Antemortem

Recipients

Controls

Animal ID

Rectal tissues

Rectal tissues

Other lymphoid tissuesc

Brains

PBMC

4

6

12

18

21

10

25

10

25

10

25

15

17#

1

4454

PBMC

1.3 × 108

VRQ/VRQ

 

4599

(−)

(−)

NA

NA

NA

(+)

NA

(+)

NA

(−)

NA

NA

NA

    

VRQ/VRQ

 

4601

(−)

(+)

NA

NA

NA

(+)

NA

(+)

NA

(−)

NA

NA

NA

 

4438

 

4.5 × 108

VRQ/VRQ

 

4598

(−)

(−)

NA

NA

NA

(−)

NA

(+)

NA

(−)

NA

NA

NA

    

VRQ/VRQ

 

4600

(−)

(−)

NA

NA

NA

(+)

NA

(+)

NA

(−)

NA

NA

NA

   

NA

 

VRQ/VRQ

4602

(−)

(−)

NA

NA

NA

(−)

NA

(−)

(−)

(−)

NA

NA

NA

     

VRQ/VRQ

4603

(−)

(−)

NA

NA

NA

(−)

NA

(−)

(−)

(−)

NA

NA

NA

2

4454

Monocytes

2.2 × 106

VRQ/VRQ

 

4594

(−)

ND

(−)

(−)

(−)

NA

(−)

NA

(−)

NA

(−)

(−)

ND

    

VRQ/VRQ

 

4595

(−)

ND

(+)

(+)

(+)

NA

(+)

NA

(+)

NA

(+)

(P)

ND

 

4438

 

4.5 × 106

ARQ/VRQ

 

4546

(−)

ND

(−)

(−)

(−)

NA

(−)

NA

(−)

NA

(−)

(−)

ND

    

VRQ/VRQ

 

4593

(−)

ND

(−)

(−)

(−)

NA

(−)

NA

(−)

NA

(−)

(−)

ND

    

VRQ/VRQ

 

4597

(−)

ND

(+)

(+)

(+)

NA

(+)

NA

(+)

NA

(+)

(P)

ND

   

NA

 

VRQ/VRQ

4596

(−)

ND

(−)

(−)

ND

NA

(−)

NA

(−)

NA

(−)

(−)

ND

3

4454

T lymphocytes

5.9 × 106

VRQ/VRQ

 

4539

(−)

ND

(−)

(−)

(−)

NA

(−)

NA

(−)

NA

(−)

ND

(−)

    

VRQ/VRQ

 

4590

(−)

ND

(−)

(+)

(+)

NA

(+)

NA

(+)

NA

(+)

ND

(P)

 

4438

 

9.9 × 106

VRQ/VRQ

 

4588

(−)

ND

(−)

(−)

(−)

NA

(−)

NA

(−)

NA

(−)

ND

(−)

    

VRQ/VRQ

 

4589

(−)

ND

(−)

(−)

(−)

NA

(−)

NA

(−)

NA

(−)

ND

(−)

   

NA

 

VRQ/VRQ

4591

(−)

ND

(−)

(−)

ND

NA

(−)

NA

(−)

NA

(−)

ND

(−)

     

VRQ/VRQ

4592

(−)

ND

(−)

(−)

ND

NA

(−)

NA

(−)

NA

(−)

ND

(−)

  1. Abbreviations: PRNP prion genotype, PBMC peripheral blood mononuclear cells, NA not applicable, ND Not done, sPMCA Serial protein misfolding cyclic amplification, P positive for prion protein misfolding activity; #months post-inoculation; (+) = PrPSc immunolabeling was detected; (−) = PrPSc immunolabeling was not detected; aindicate the total number of PBMC, monocytes or T lymphocytes isolated from a 50 mL whole blood sample volume; bPrPSc immunolabeling in the tissues were detected using a mixture of prion mAbs F99/97.6.1 and F89/160.1.5 by immunohistochemistry; cOther lymphoid tissues include alimentary tract-associated lymphoid tissues (tonsils, retropharyngeal, mesenteric such as ruminal, abomasal, duodenal, jejunal, ileocecal, distal ileum, ileocecal junction lymph nodes, ileum, and spleens) and peripheral lymph nodes (prescapular, prefemoral, and popliteal)