Fig 5

Anticoccidial activity of intracellular proteins. After 24 h of incubation with the intracellular crude extract, 61 % of E. tenella oocysts maintained their integrity in the intracellular crude extract, while in the protein precipitated with a 40 % fraction precipitated with ammonium sulfate (AS) 61.74 % of oocysts remained its integrity. After 24 h of incubation, 40 % precipitated with AS and treated with trypsin showed 80.92 % of integrity. The percentage of integrity decreased at 48 h to 45 and 44.06 % in intracellular crude extract and 40 % fraction precipitated with AS, respectively, whereas in the 40 % fraction treated with trypsin the integrity was 80.92 and 87.86 % at 24 and 48 h, respectively. . Significant differences among control and 24 h are shown with an asterisk while significant differences among control and 48 h and control are shown with double asterisk