Table 1 Primers used in QRT-PCR analysis
Gene | Forward primer | Reverse primer | Probe primer | Opt. primer conc. | Probe (μM) | GenBank |
|---|---|---|---|---|---|---|
28S | GGCGAAGCCAGAGGAAACT | GAC GACCGATTTGCACGTC | AGGACCGCTACGGACCTCCACCA | 0.6 | 5 | FM165415 |
C1S | GCGCAAAGGCTGGAAAATAC | TCAAGAACAGAATTGGGAGTGACA | TACTATGCTGAACCCATAACCTGTCTCCCG | 0.6 | 5 | NM_001030777 |
CCL13 | CAGAGCCTGGCCCAGAGA | TGTCCATTTTGATTCTTCTGGTATG | CTGTGCCTGACAAGTGCTGCTTCAACTT | 0.2 | 5 | XM_415779 |
CCLi7 (ah221) | CACAACCTGCTGCTTCTCCTATG | TGTAGGCGGAGGCAATGAG | TCAACGTCCCGTCCCACGCA | 0.2 | 5 | AY037860 |
CD38 | GCTTGATGGGCTTTCATGGT | CACATTCACTCCATTTTGGACAA | ACCCCTCAGCTCCAGGAATCAACTATGAA | 0.6 | 5 | XM_420774 |
CLU | TGAGTCAGAATCCCGTAACTTCAG | GCAGTCCACAGCCAAGATCTC | AGATCCGGCGCAACTCGGCC | 0.2 | 5 | NM_204900 |
COX11 | TGGGATCTCCACCTACAACGTA | CAGCCACTGTTCTTCAAAACAAA | TGCCCTTCGAAGCAGGACAGTACTTCA | 0.4 | 5 | XM_001233972 |
DDT | GGCCCCGAGCGGATT | CATGACTGTTCTGTTCTTGCCAAT | CATTCGCTTTTACCCGCTGGAGCC | 0.8 | 5 | NM_001030667 |
FK-506-BP51 | CGGAGGATCAAGAGGAAAGGA | CAGAACCCCTCCAGGTGAATT | AAGGCTATTCCAACCCCAACGAAGGTG | 0.1 | 5 | NM_001005431 |
HSC20 | GGAAATCATGGAAATCAATGAGAAA | CACCTCTTTGGTCAGTTCTTCTTG | CAGAGCCCGAGAACGACGAGATCC | 0.8 | 5 | XR_026662 |
IFNAR2 | TGGTCACTGCATCTCTAAATAAACATT | CTGCAATTGTGATGCCATAATAATC | CATCCCATCAGCCTGGAAATGCATAACT | 0.4 | 5 | NM_204858 |
IGFBP5 | GAAGAGCAGCCAGAGGATGGT | GCTTGCACTGCTTCCTCTTGT | CACCTCCCCAACTGCGACCGAAA | 0.1 | 5 | XM_422069 |
IRF-7 | ACCCGGACCGCCGTAT | GCCCAGGCCTTGAAGATCTC | CATCCCTTGGAAGCACAACGCCA | 0.6 | 5 | NM_205372 |
MAFK | GCGATGATGAACTCGTGTCAA | TTCAGACGGATGACCTCCTCTT | TCCGTACGGGAGCTGAACCAGCAC | 0.4 | 5 | NM_204756 |
MAP4K4 | TGCTATTGAAATGGCTGAAGGA | TCCGTGGGATGAGGAAGAGT | TCCTCCCCTGTGTGACATGCACCC | 0.2 | 5 | NM_001031126 |
MMD2 | TGCCACGCACGCATTCT | GGTCATCGGAGAGGACGTAGAG | TCCTGCCCAGCATCCTCGGC | 0.4 | 5 | XM_414787 |
MX1 | TGGACTTCTGCAACGAATTGTC | ATCCAGAAGAGTGCTGAAATGTTTG | TTCACCTCCGCAATCCAGCAAGAGA | 0.6 | 5 | NM_204609 |
SRI | TACTATCAGGGCGGGTATGGA | AGCAAAATAACCATACAGAGGATCCT | CAGCTCCAGGAGGCCCATCATTCC | 0.1 | 5 | NM_001080865 |
SUCLG2 | AGCTTCCCGGCTGTTCAAT | CATGGTCTGCCATCAGCTTTT | AACCCCTAGACGATGGCTGAATCTGCA | 0.1 | 5 | NM_001006141 |
TLR3 | ATCCATGGTGCAGGAAGTTTAAG | GATGGAGTCTCGACTTTGCTCAATA | TGCATCATGCTTTACAGC | 1.0 | 5 | JF273967.1 |
TNFAIP1 | GTTGTGGGAAGCACTTTGGAA | TCAACTCCTTGATCTCCTGTCTGT | CCGAGATGACACAATTGCACTTCCAAAA | 0.2 | 5 | NM_001030726 |
TP-D53 | TGGCCAAGCTAGAGGATGAAA | TCAGGCTCATGCCAAGTTTTT | ACTAGCAGCCAAAGAAAAGCACCTGATTGA | 0.2 | 5 | NM_204215 |