Fig. 6

SG2000-induced DNA interstrand cross-linking, expressed as the % decrease in comet tail moment (% DTM) in tumour cells (solid bars) from mice carrying either a) LMeC or b) CMeC-1 subcutaneous xenografts. Samples were harvested at 2 and 24 h following treatment with either 0.15 mg/kg or 0.30 mg/kg (single dose). Values are the mean % DTM ± SEM calculated from 50 tumour cells each, from three mice each at each time point. In b) open bars are the % DTM in peripheral blood mononuclear cells taken at the same time points as the CMeC-1 tumours. c. SG2000-induced DNA interstrand cross-linking in peripheral blood mononuclear cells from mice carrying LMeC or CMeC-1 xenografts. Blood samples were harvested from at least three mice at 2 h and from at least three mice at 24 h, following each of three once-weekly doses of SG2000 (either 0.15 mg/kg or 0.30 mg/kg). The % DTM was calculated from 50 cells in each sample as described previously and data are the mean ± SEM. Statistical analysis: p ≤ 0.001 = ***, p ≤ 0.01 = **, p ≤ 0.05 = *, ns = not significant