Fig. 3From: A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus E. coli protein contamination in the purified antigen (Ag) induces background signal in an indirect ELISA (panel a). This background is reduced in a sandwich ELISA using a mouse monoclonal anti-his antibody as a capture antibody for the target antigen (panel b-d). Pooled EEHV-gB peptide specific rabbit serum was used in a 1:200 dilution. Panel A no capture antibody, panel B 100 ng capture antibody, panel c 200 ng capture antibody, panel D 300 ng capture antibodyBack to article page