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Fig. 3 | BMC Veterinary Research

Fig. 3

From: A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus

Fig. 3

E. coli protein contamination in the purified antigen (Ag) induces background signal in an indirect ELISA (panel a). This background is reduced in a sandwich ELISA using a mouse monoclonal anti-his antibody as a capture antibody for the target antigen (panel b-d). Pooled EEHV-gB peptide specific rabbit serum was used in a 1:200 dilution. Panel A no capture antibody, panel B 100 ng capture antibody, panel c 200 ng capture antibody, panel D 300 ng capture antibody

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