Fig. 6From: Inhibition of porcine reproductive and respiratory syndrome virus replication in vitro using DNA-based short antisense oligonucleotidesIndirect immunofluorescence detection of PRRSV in MARC-145 cells transfected with or without AS-ONs. Transfection was performed 90 min post infection with PRRSV YN-1 strain (25 TCID50). Cells were fixed 60 h post transfection, reacted against anti-N monoclonal antibody (MAb) and then incubated with fluoresce in Alexa Fluo488 conjugated secondary antibody. Blue color stains nuclei to show the total number of cells. The green color indicates the expression of N protein in MARC-145 cells. The images of the same treatment from the two channels were merged. Treatment with YN8, 5UP2*, YN4 and YN5 resulted in reduction of virus replication, while 5UP2 and the other AS-ONs did not appear to have obvious effects under identical conditionsBack to article page