Detection and molecular characterisation of bovine corona and toroviruses from Croatian cattle

BMC Veterinary Research

Table 1 Oligonucleotide primers for PCR and sequencing reaction

PRIMER NAME	SEQUENCE (5’ → 3’)	SIZE (bp) and location of amplicon	GENOME POSITION^a	REFERENCE
BCoV F	CCGATCAGTCCGACCAATC	460	29476–29494	[46]
BCoV R	TAGTCGGAATAGCCTCATCGC	BCoV N gene	29899–29919	[46]
S-S1	GATAAGTTTGCTATACCCAATGG	1194	24817–24839	[47]
S-AS1	ACTATCATTTACTGAATTAACAG	BCoV S gene	25988–26010	[47]
TORO S5	GTGTTAAGTTTGTGCAAAAAT	741	20956–20976	[5]
TORO S3	TGCATGAACTCTATATGGTGT	BToV S gene	21677–21697	[5]

^aNumbering according to the complete genome of BCoV strain Mebus (U00735) and BToV strain Breda 1 (AY427789)

ISSN: 1746-6148