Fig. 3

SYBR Green real-time RT-PCR and conventional RT-PCR detection of HEV viral RNA in serum samples from chickens challenged with CaHEV. In the linear graph (bottom), viral RNA in serum samples was quantitatively detected using the SYBR Green real-time RT-PCR. Each data point represents the mean value (±SD) generated from three replicates. The line graph plotted above depicts qualitative detection of avian HEV RNA by conventional RT-PCR. A “-” represents a negative sample and a “+” represents a positive sample