Table 2 Detection of Borrelia sp DNA in lungs and heart of rodents using LightMix® and ‘’in house” nested PCR, both targeting ospA gene
From: Detection, identification and genotyping of Borrellia spp. in rodents in Slovenia by PCR and culture
Findings | Sample | Apodemus flavicollis | Myodes glareolus | Apodemus sylvaticus | Apodemus flavicollis | Myodes glareolus | Neomys anomalus | Crocidura suaveolens |
|---|---|---|---|---|---|---|---|---|
Retrospective (year 2012) | Prospective (year 2013) | |||||||
ospA pos | Lungs | ND | 1 | 6 | 0 | 0 | ||
LightMix pos | heart | 13 | 17 | 2 | 9 | 8 | 0 | 0 |
ospA neg | Lungs | ND | 13 | 15 | 5 | 1 | ||
LightMix neg | heart | 114 | 63 | 0 | 9 | 13 | 5 | 1 |
ospA neg | Lungs | ND | 3 | 0 | 0 | 0 | ||
LightMix pos | heart | 28 | 14 | 0 | 0 | 0 | 0 | 0 |
ospA pos | Lungs | ND | 0 | 1 | 0 | 0 | ||
LightMix neg | heart | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
All | Lungs | ND | 18 | 22 | 5 | 1 | ||
heart | 155 | 94 | 2 | 18 | 22 | 5 | 1 | |