Fig. 1

Cancer cells inhibit LPS induced activation of macrophages. Macrophages (RAW) were co-cultured with either canine mammary carcinoma (REM), Human mammary cancer cells (MCF7) or non-cancerous cells (HEK293T), stimulated with 1 μg/ml LPS for 48 h. LPS-induced activation of macrophages was measured by changes in granularity (a (i)), percentage of MHC II+ cells (a (ii)) or mean fluorescence intensity of MHC II (a (iii)), and expression of the M2 marker, CD301 (b). Macrophages were distinguished from cancer cells in flow cytometry by CSFE staining. All experiments are replicates of 4. Asterisks indicate statistically significant differences in relation to LPS-treated cells, where P < 0.05 (unless where indicated) by Kruskal-Wallis test; “ns” indicates non-significant differences