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Table 8 Systematic trimming of the frontoparietal region (Block B)

From: International veterinary epilepsy task force recommendations for systematic sampling and processing of brains from epileptic dogs and cats

Cuts

Time

View/specimen

Landmarks and cutting levels

Orientation of sections

Aim/harvest

Difficulty

TS-4

13 min

Ventral view of the frontal lobe

Transverse line through or just rostral to optic chiasm

2D knife axis: laterolateral.

Standard section of the frontal lobe.

Easy

Plane: transverse.

Boundary between thalamus and basal nuclei; also shows septal nuclei, body of fornix, rostral commissure parietofrontal cortex.

Blade movement: ventrodorsal.

TS-5

15 min

Ventral (or dorsal) view of the frontal lobe

Transverse midline section of olfactory tuberculum

2D knife axis: laterolateral.

Standard section of the frontal lobe providing the best view of the basal nuclei and capsules

Easy

Plane: transverse.

Blade movement: ventrodorsal.

HOR-2

17 min

Rostral view of the still connected hemispheres of olfactoryfrontal brain

2: Horizontal midline section through proreus gyrus.

2D knife axis: laterolateral.

Epilepsy-specific section of the susceptible olfactoryfrontal cortex.

Easy

2′,2″:..followed by parallel sections of the ventral block with 3 mm slice thickness.

Plane: horizontal.

Standard section in ethmoidal pathologies.

Blade movement: rostrocaudal.

SAG-1L/R

19 min

Rostral view of the dorsal block of the olfactoryfrontal brain

1L/R: Sagittal lines through lateral third of proreus gyrus.

2D knife axis: rostrocaudal.

Epilepsy-specific sections exposing motor cortex

Easy

1 L′/R′/1 L″/R″:..followed by parallel sections with 3 mm slice thickness

Plane: sagittal.

Blade movement: rostrodorsal to caudoventral.