Table 1 PCR primers used in this study
Primer name | Primer sequence (5′ → 3′) | Application |
|---|---|---|
GP5-ORF F | ACTTAAGCTTGGTACCATGG | Amplification of GP5 ORF |
GP5-ORF R | CGCTAGAGCGCTGGCAAGTG | Amplification of GP5 ORF |
GP5a F | CCGGGATCCAACGCCAGCGACAACAAC | Amplification of GP5a fragment |
GP5a R | CACCGCCGCTTCCACCGCCACCCTCCACTGCCCAGTCAAA | Amplification of GP5a fragment |
GP5b F | GTGGAAGCGGCGGTGGCGGAAGCTGCATGTCCTGGCGCTA | Amplification of GP5b fragment |
GP5b R | CCGGAATTCGAGACGACCCCATAGTTCCGCT | Amplification of GP5b fragment |
fljB F | GGGGGAATTC GGTGGCGGTGGTTCTATGGCACAAGTAATCAACACTAACAGT | Amplification of fljB fragment |
fljB R | GCGTCGACTTAACGTAACAGAGACAGC | Amplification of fljB fragment |