Table 1 Primers in this work
Primer | Primer sequences (5′–3′) | Restriction site |
|---|---|---|
LH21 | GTCCTGGCTGCTCTTCTACAAGG | None |
LH22 | GGTACGTGCTGTTGAACTGTTCC | None |
VHU | GCCATGGCTSAKGTBCAGCTGSTGGAGTCTGG | None |
VHD | GCGGCTGAGGAGACGGTGACCWGG | None |
VHHSfiI | ATTACTGGCCCAGCCGGCCGGCTGAKGTBCAGCTGGTGGAGTCTGGRGGAGGCT | SfiI |
VHHNotI | ATTATTAGTGCGGCCGCTGAGGAGACGGTGACCWGGGTCC | NotI |
D01U | CTTGGTCTCTGATGTGCAGCTGGTGGAGTC | BsaI |
D01D | CTCGGATCCTCATGAGGAGACAGTGACCTGG | BamHI |
A01U | CTTGGTCTCTGATGTGCAGCTGGTGGAGTC | BsaI |
A01D | CTCGGATCCTCATGAGGAGACGGTGACCTGG | BamHI |