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Table 4 Results of RT-qPCR to evaluate in vitro expression of transgenes from MIDGE-Th1 vectors encoding eqIL12, eqIL18, htyr and hgp100

From: Local and systemic effect of transfection-reagent formulated DNA vectors on equine melanoma

MIDGE-Th1

Estimated mRNA / cDNA copy numbers per transgene (Ct values)

vectors [μg]

eqIL12-p35

eqIL12-p40

eqIL18

htyr

hgp100

eqIL12, eqIL18

1.14 + E03

1.52 + E04

3.16 + E03

< LOD

< LOD

[0.5 + 0.5]

(27)

(23)

(25)

eqIL12, eqIL18, htyr

1.01 + E03

2.10 + E04

5.23 + E03

3.01 + E04

< LOD

[0.5 + 0.5 + 1.25]

(28)

(23)

(25)

(22)

eqIL12, eqIL18,

9.10 + E02

9.79 + E03

1.98 + E03

< LOD

1.30 + E04

gp100

(28)

(24)

(26)

 

(23)

[0.5 + 0.5 + 1.25]

  1. LOD (limit of detection): = < 25 copies/reaction.