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Table 2 Statistical analysis of reactive oxygen species generation by blood neutrophils following opsonized zymosan particles stimulation

From: Reactive oxygen species generation by bovine blood neutrophils with different CXCR1 (IL8RA) genotype following Interleukin-8 incubation

Polymorphism 1

Fixed effect

n 2

AUC 3

RLUmax 4

Tmax 5

β 6

SE 7

P 8

β 6

SE 7

P 8

β 6

SE 7

P 8

c.735C > G

Intercept

 

253

32

 

69

10

 

31.7

4.0

 
 

Genotype

   

0.40

  

0.82

  

0.22

 

 c.735CC

7

Ref.9

 

Ref.9

 

Ref.9

 
 

 c.735CG

6

-36

47

 

-6

14

 

-3.6

5.3

 
 

 c.735GG

7

-63

45

 

-9

14

 

-9.2

5.1

 
 

Incubation

   

<0.01

  

<0.01

  

0.54

 

 2 h

 

Ref.9

 

Ref.9

 

Ref.9

 
 

 6 h

 

20

7

 

5

2

 

1.2

1.9

 
 

rbIL-8

   

<0.01

  

<0.01

  

0.89

 

 0 ng/ml

 

Ref.9

 

Ref.9

 

Ref.9

 
 

 40 ng/ml

 

-6

8

 

-3

2

 

-0.6

2.3

 
 

 400 ng/ml

 

-26

8

 

-8

2

 

0.5

2.3

 

c.980A > G

Intercept

 

248

48

 

63

15

 

32.9

6.0

 
 

Genotype

   

0.32

  

0.58

  

0.44

 

 c.980AA

3

Ref.9

 

Ref.9

 

Ref.9

 
 

 c.980AG

5

13

61

 

12

18

 

-3.6

7.3

 
 

 c.980GG

12

-51

54

 

-2

16

 

-7.8

6.4

 
 

Incubation

   

<0.01

  

<0.01

  

0.54

 

 2 h

 

Ref.9

 

Ref.9

 

Ref.9

 
 

 6 h

 

20

7

 

5

2

 

1.2

1.9

 
 

rbIL-8

   

<0.01

  

0.17

  

0.89

 

 0 ng/ml

 

Ref.9

 

Ref.9

 

Ref.9

 
 

 40 ng/ml

 

-6

8

 

-3

2

 

-0.6

2.3

 
 

 400 ng/ml

 

-26

8

 

-8

2

 

0.5

2.3

 
  1. 1Linear mixed regression models describing the association between reactive oxygen species generation by blood neutrophils and CXCR1 polymorphisms c.735C > G and c.980A > G, respectively. Neutrophils were incubated with 0, 40 or 400 ng recombinant bovine interleukin 8 per mL for 2 or 6 h and stimulated with opsonized zymosan particles,
  2. 2Number of heifers,
  3. 3Area under the curve values in 106 RLU (relative light units) * s,
  4. 4Peak values in 103 RLU
  5. 5Time of peak values in min,
  6. 6Regression coefficient,
  7. 7Standard error,
  8. 8Overall P-value of the fixed effect,
  9. 9Reference,
  10. All two-way interactions between the fixed effects were non-significant (P > 0.05) and removed from the model.