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Figure 7 | BMC Veterinary Research

Figure 7

From: RhoA/ROCK1 regulates Avian Reovirus S1133-induced switch from autophagy to apoptosis

Figure 7

Functional interaction between RhoA, ROCK and Beclin-1 in ARV S1133-infected cells (A, B). Vero cells pretreated with DMSO (left panel) or Y-27632 (right panel) for 4 hr then infected with ARV S1133 at an MOI of 5 for various incubation periods. 500 μg soluble cell lysates were precleared and immunoprecipitated with IP antibodies as indicated. The immunocomplexes were purified by protein A beads and loaded onto an SDS-PAGE gel. The interacting proteins were detected using the Western blotting technique.

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