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Figure 2 | BMC Veterinary Research

Figure 2

From: RhoA/ROCK1 regulates Avian Reovirus S1133-induced switch from autophagy to apoptosis

Figure 2

Upregulation of autophagic and apoptotic effectors and the Beclin-1 promoter by ARV S1133. (A) Vero cells infected with ARV S1133 at an MOI of 5 (B) DF1 cells infected with ARV S1133 at an MOI of 20. At the indicated time points, total cell lysates were collected. 30 μg of the extracted protein was separated by SDS-PAGE and transferred to a PVDF membrane. The expression of specific proteins was detected using the indicated antibodies. (C) Luciferase assay. Vero cells were transfected with three luciferase reporters with different lengths of the 5′-end regulatory region of the Beclin-1 gene. Following incubation with ARV S1133 for different time periods, the luciferase and β-galatosidase activities were measured and normalized to the transfection efficiency. The promoter activation levels were calculated against time zero. All experiments were performed three times, each in duplicate. The data are presented as the mean ± standard deviation (SD).

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