Expression of genes involved in the T cell signalling pathway in circulating immune cells of cattle 24 months following oral challenge with Bovine Amyloidotic Spongiform Encephalopathy (BASE)

Table 1 Primer used for qPCR

Gene	Function	Sequence
TCR delta chain	Antigen receptor of T cells	Forward 5′TCGCTTGTTTGGTGAAGGA
TCR delta chain	Antigen receptor of T cells	Reverse 5′CCCAGGTGAGATGGCAATAG
TRAT1	TCR associated membrane adapter 1: TCR-mediated T cell activation cascade	Forward 5′GTGAACAAACTGCAAGACGC
TRAT1		Reverse 5′CTGGGCTTTCTTCGCTTCC
CD3E	Marker of thymocytes and peripheral T lymphocytes	Forward 5′TCTGGGACTCTGCCTCTTATTA
CD3E	Marker of thymocytes and peripheral T lymphocytes	Reverse 5′CAAACTCTCTAGGGCATGTCAG
LAT	Linker for activation of T cells, transduction of the activation signal downstream CD3	Forward 5′GGAGTCGGGAATATGTGAATGT
LAT		Reverse 5′CTGGGAATTCTGGGTGTCAG
ZAP70	Associated with CD3Z chain; transduction of the activation signal downstream CD3	Forward 5′CTCATGGCTGACATCGAACT
ZAP70		Reverse 5′CCACGTCGATCTGCTTCTT
LCK	lymphocyte-specific protein tyrosine kinase	Forward 5′GACAGCACCAGAAGCCATTA
LCK	lymphocyte-specific protein tyrosine kinase	Reverse 5′GCGACCATGAGTGACAATCT
B2MG	Beta-2-microglobulin precursor	Forward 5′CAGCGTCCTCCAAAGATTCA
B2MG	Beta-2-microglobulin precursor	Reverse 5′ACCCATACACATAGCAGTTCAG
ACTB	Beta-Actin	Forward 5′AGTCCTTTGCCTTCCCAAAA
ACTB	Beta-Actin	Reverse 5′AAGCGATCACCTCCCCTGT

ISSN: 1746-6148