Figure 2From: One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasisSignature analysis of secreted cytokine by peripheral blood leukocytes afterin vitrostimulation withLeishmania infantum soluble antigens (SLAg). (A) Establishment of the global median cut-off edges for each cytokine (IL-8, TNF-α, IFN-γ, IL-4 and IL-10) used to segregate dogs as they present “Low” () or “High” () cytokine levels. (B) Gray-scale diagrams used to compile the frequency (%) of high cytokine producers. (C) Ascendant cytokine signatures were assembled for each time after vaccination (T0, T1, T6 and T12). The frequencies of high producers were considered relevant (*) when the percentage was confined over the 50th percentile (doted lines). (D) Comparative analysis of cytokine signatures were used to identify relevant differences amongst Leishmune® vaccinated dogs at one (T1 = black rectangle), six (T6 = dark grey rectangle) and twelve (T12 = light grey rectangle) months post-vaccination compared to unvaccinated dogs (T0 = white rectangle). Gray scale rectangles were used to highlight the shift in the overall profile of pro-inflammatory and regulatory cytokines on each time after immunization. Relevant differences (shift of frequencies across the 50th percentile cut-off edge) were highlighted by gray background.Back to article page