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Table 2 Primers used in PCR to identify species and subspecies of Salmonella strains, according to Lee et al. [13]

From: Prevalence and genetic characteristics of Salmonella in free-living birds in Poland

Genes

Function of gene

Sequence of nucleotides

Size

STM

encodes a putative inner membrane protein, specific for S. enterica subsp I

F-GGTGGCCTCGATGATTCCCG

137 bp

R-CCCACTTGTAGCGAGCGCCG

stn

encodes Salmonella enterotoxin and is specific for S. enterica

F-CGATCCCTTTCCCGCTATC

179 bp

R-GGCGAATGAGACGCTTAAG

invA

invasion protein, for simultaneous identification of Salmonella at the genus level

F-ACAGTGCTCGTTTACGACCTGAAT

244 bp

R-AGACGACTGGTACTGATCGATAAT

gatD

encodes the galacitol-1-phosphate dehydrogenase (gatD), contributes to acid production from galacitol

F-GGCGCCATTATTATCCTATTAC

501 bp

R-CATTTCCCGGCTATTACAGGTAT

mdcA

encodes the alpha subunit of the enzyme that contributes to malonate utilization

F-GGATGTACTCTTCCATCCCCAGT

728 bp

R-CGTAGCGAGCATCTGGATATCTTT

fljB

encodes phase 2 flagellin, enables differentiation between monophasic and diphasic subspecies

F-GACTCCATCCAGGCTGAAATCAC

848 bp

R-CGGCTTTGCTGGCATTGTAG