Development of a nanoparticle-assisted PCR (nanoPCR) assay for detection of mink enteritis virus (MEV) and genetic characterization of the NS1 gene in four Chinese MEV strains

Table 2 NanoPCR and conventional PCR target gene and primers used for amplification of MEV

Primer name ^a	Length (nt)	Genome position ^b	Sequence (5′-3′)	Melting temperature (°C)	Product (bp)
P1	20	1906-1925	ACAAGCGGCAAGCAATCCTC	54.9	194
P2	20	2080-2099	CTGCCTCTATTTCGGACCAT
P3	23	151-173	CGCCATGTCTGGCAACCAGTATA	56	2013
P4	25	2139-2163	GGTTAATCCAAGTCGTCTCGAAAAT	56	2013

^aP1 and P2 were used to amplify a portion of the NS1 gene (194 bp). P3 and P4 were used to amplify the full-length MEV NS1 gene (2,013 bp).
^bThe nucleotide positions of the nanoPCR and conventional PCR primers are according the genome sequence of mink enteritis virus strain MEVB (GenBank accession number FJ592174).

ISSN: 1746-6148