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Figure 1 | BMC Veterinary Research

Figure 1

From: 6-Thioguanine and zebularine down-regulate DNMT1 and globally demethylate canine malignant lymphoid cells

Figure 1

Western blot of DNMT1 expression. This image is a western blot of DNMT1 protein expression in CLGL 90 and CLL 17–7 cells treated with 1.5 μM or 6 μM 6-TG or 50 μM or 200 μM Zeb concentrations when compared to control. DNMT bands are evident at approximately 180,000 molecular weight. The treated groups had a reduction in intensity of DNMT1 expression as evident by bands in the 180 kDA region. Reduction in the expression of DNMT1 treated CLGL 90 cells (6-TG low, high and Zeb low, high groups) was 0.81, 0.76, 0.89, and 0.67 when compared to untreated control, respectively. Reduction in the expression of DNMT1 treated CLL 17–7 cells (6-TG and Zeb high dose) is 0.27 and 0.40, respectively. Loading control is Beta-actin.

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