Table 1 Essential parameters and figures-of-merit of the analytical method adopted for determination of cortisol, cortisone, prednisolone and some of its metabolites
Analyte | Retention time (min) | Precursor ion m/z | Product ions | CCα (ng/mL) | LOD (ng/mL) |
|---|---|---|---|---|---|
6β-hydroxyprednisolone | 4,3 | 377.3 | 377.3➔341.2 Q | -- | 0.41 |
377.3➔323.2 | |||||
377.3➔359.3 | |||||
20α-dihydroprednisolone | 10.5 | 363.3 | 363.3➔267.4 Q | -- | 0.36 |
363.3➔291.3 | |||||
363.3➔309.3 | |||||
20α-dihydroprednisolone | 11.6 | 363.3 | 363.3➔345.2 Q | -- | 0.35 |
363.3➔267.3 | |||||
363.3➔291.3 | |||||
20α-dihydroprednisone | 12.3 | 361.3 | 361.3➔153.2 Q | -- | 0.42 |
361.3➔297.3 | |||||
361.3➔313.2 | |||||
Prednisolone | 17.1 | 361.3 | 361.3➔279.2 Q | 0.67 | 0.05 |
361.3➔265.2 | |||||
361.3➔223.2 | |||||
Prednisone | 17.9 | 359.3 | 359.3➔313.2 Q | 0.66 | 0.05 |
359.3➔295.2 | |||||
359.3➔267.2 | |||||
Cortisol | 17,7 | 363.2 | 363.2➔121.3 Q | -- | 0.1 |
363.2➔147.3 | |||||
363.2➔309.4 | |||||
Cortisone | 18.9 | 361.2 | 361.2➔163.4 Q | -- | 0.1 |
361.2➔121.3 | |||||
361.2➔105.3 | |||||
Triamcinolone Acetonide D6 | 27.1 | 441.4 | 441.4➔421.3 Q | -- | -- |
441.4➔403.4 |