Figure 1
From: Mycoplasma synoviaeenolase is a plasminogen/fibronectin binding protein
Analysis of rMsEno expression and purification using SDS-PAGE followed by Coomassie blue staining. M: PageRulerâ„¢ Prestained Protein Ladder (SM0671, Fermentas). Lane 1: Total cellular proteins of E. coli BL21 (DE3) cells transformed by pET-28a (+). Lane 2: Total cellular proteins of E. coli BL21 (DE3) cells transformed by pET-Eno. Lane 3: Supernatant of lysate of E. coli BL21 (DE3) cells transformed by pET-Eno. Lane 4: Sediment of lysate of E. coli BL21 (DE3) cells transformed by pET-Eno. Lane 5: Purified recombinant protein.