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Table 4 Primers and probes used in cloning, CDV RT-iiPCR and real-time RT-PCR

From: Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction

Primer

Nucleotide sequence (5'-3')

Note*

CDV-F

AGCTAGTTTCATCTTAACTATCAAATT

CDV real-time RT-PCR forward primera

CDV-R

TTAACTCTCCAGAAAACTCATGC

CDV real-time RT-PCR reverse primera

CDV-P

FAM-ACCCAAGAGCCGGATACATAGTTTCAATGC-BHQ1

CDV real-time RT-PCR probea

CDV-F1

ATGGCTAGCCTTCTTAAAAGCCTCACA

CDV cloning primer (nt108-134)*

CDV-R1

TTCCGATCATCGTCATTTCCATCA

CDV cloning primer (nt1570-1547)*

CDViiF

CCGGAAATCAACGGACCTAAATT

CDV RT-iiPCR primer (nt291 ~ 313)*

CDViiR

GTCGTCTATGATCCTCTGGATCAA

CDV RT-iiPCR primer (nt389 ~ 366)*

CDViiP

FAM-CAGTATCCTCTCCTTGTTCGTGGA-MGB-NFQ

CDV RT-iiPCR probe (nt329 ~ 352)*

  1. *Nucleotide position is based on GenBank accession no. AF305419.1; aElia et al. [42].