Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction

Table 2 Exclusivity analysis of CDV RT-iiPCR

Pathogen	Source	RT-iiPCR		Real-time RT PCR (Ct)
Pathogen	Source	Results	S/N	Real-time RT PCR (Ct)
Canine distemper virus	Snyder Hill strain (ATCC)	+	1.94	34.45
Bordetella bronchiseptica	Clinical sample*	-	0.93	Neg
Canine herpesvirus	ATCC strain	-	0.97	Neg
Canine respiratory coronavirus	Clinical sample*	-	0.90	Neg
Canine parainfluenza virus	ATCC strain	-	0.93	Neg
Canine adenovirus 2	ATCC strain	-	0.93	Neg
Canine parvovirus	Cornell strain	-	0.94	Neg
Influenza virus H3N8	Clinical sample*	-	0.93	Neg

*Pathogens obtained from clinical samples were previously cultured/isolated and validated by sequencing by the UTCVM Clinical Bacteriology and Virology Labs.

ISSN: 1746-6148