Table 6 Characteristics of the primers used in RT-PCR analysis and PCR product sizes
From: Studies on the health impact of Agrimonia procerain piglets
Gene | Forward primer (from 5' to 3') | Product size (bp) | NCBI GenBank number |
|---|---|---|---|
Reverse primer (from 5'to 3') | |||
β-actin | GACATCCGCAAGGACCTCTA | 205 | DQ_845171.1 |
ACATCTGCTGGAAGGTGGAC | |||
SDHA1 | CTAGCCCCCGTCGCAAAGG | 380 | DQ_402993 |
AGTTTGCCCCCAGGCGGTTG | |||
RPP0 | CAACCCTGAAGTGCTTGACA | 204 | NM_001098598.1 |
GCCTTGACCTTTTCAGCAAG | |||
TNFα | AACCCTCTGGCCCAAGGA | 57 | NM_214022.1 |
GGCGACGGGCTTATCTGA | |||
IL-1β | AAAGGGGACTTGAAGAGAG | 286 | NM_001005149.1 |
CTGCTTGAGAGGTGCTGATGT | |||
IL-10 | GCATCCACTTCCCAACCA | 446 | NM_214041.1 |
CTTCCTCATCTTCATCGTCAT |