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Figure 5 | BMC Veterinary Research

Figure 5

From: Brucella cetiinfection in dolphins from the Western Mediterranean sea

Figure 5

Molecular characterization of Brucella isolates by PCR-RFLP of omp2b locus and Multiplex PCR. Panel A: PCR-RFLP of omp2b locus, showing a common haplotype for the three B. ceti Mediterranean isolates (lanes 6–8), shared with that identified in all B. ceti Atlantic dolphin strains, irrespective of their geographic origin (lanes 4 and 5), but different from B. pinnipedialis and B. ceti porpoise reference (lanes 2 and 3, respectively) haplotypes. Panel B. Multiplex PCR using two primer pairs, identifying Mediterranean isolates as similar to those of Atlantic dolphin origin. Lane 1, 100 bp DNA ladder (Invitrogen Ltd.); lane 2, B. pinnipedialis B2/94 reference seal strain; lane 3, B. ceti B1/94 porpoise reference strain; lane 4, B. ceti B14/94 reference strain isolated from Atlantic dolphin; lane 5, B. ceti C1 strain isolated from a dolphin in the Cantabric sea (North Spain); lanes 6, 7 and 8, B. ceti Mediterranean isolates (bmarMR24, bmarMR25 and bmarMR26, respectively).

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