Figure 3
From: Alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation
Alkaline phosphatase mRNA analysis from bovine tissues by RT- PCR. Bovine tissues examined by RT-PCR to determine expression of genes for AP. The presence of a single 500 bp DNA fragment corresponding to the predicted product size was visualised when using (a) non-specific AP primers and (b) intestinal AP primers. PCR products were present in nasal mucosa only when non-specific AP primers were used. M = Marker (100 bp DNA ladder); MQ = high purity water; B = blank; N = nasal mucosa; L = liver; I = intestine.