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Figure 3 | BMC Veterinary Research

Figure 3

From: The different molecular forms of urine neutrophil gelatinase-associated lipocalin present in dogs with urinary diseases

Figure 3

Western blot analysis of the various NGAL molecular forms. Urine samples representing the PYU (lane 1, 5), AKI (lane 2), non-RP (enteritis, lane 3), CKD (lane 4), and control (lane 6) groups were resolved using non-reducing SDS-PAGE followed by Western blot analysis using polyclonal antibodies against canine NGAL (A), or canine MMP-9 (B). Three different NGAL related proteins, as indicated by the arrowheads, were recognized by the anti-NGAL antibodies; the protein with highest molecular weight could also be detected using the anti-MMP-9 antibodies. M, D, and C indicate the monomeric NGAL, the dimeric NGAL, and the NGAL/MMP-9 complex, respectively.

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