Figure 3

Quantification of porcine EGF secreted by L. lactis . A) Western blot analysis shows that the 6.0-kD porcine EGF was present in the supernatant of the induced LL-pEGF fermentation cultures, and no secreted pEGF was detected in the supernatant of the non-induced LL-pEGF fermentation cultures (NI). B) A representative western blot of supernatants from the induced LL-pEGF. Supernatants of the LL-pEGF induced at different times were used to determine the amounts of pEGF present in the culture supernatant by densitometry from three experiments. The pEGF concentrations in the LL-pEGF culture were about 600 ng/ml, 900 ng/ml and 1000 ng/ml at 4 h, 12 h, and 24 h, respectively. rhEGF, recombinant porcine epidermal growth factor; NI, non-induced LL-pEGF fermentation cultures.