Table 1 Sources, pre-treatments and dilutions of the antibodies used in this study
Antibody | Source | Clone | Pre-treatment | Dilution |
|---|---|---|---|---|
Anti-oestrogen receptor | Zymed / Life Technologies, USA | Poly rabbit | Nuclear Decloaker. Biocare Medical. Concord. CA. USA. pH 9.5. 3× 5 min. | 1:200 |
Anti-progesterone receptor | Immunotech SAS, Marseille, France | 10A9 rabbit | Boil in citrate buffer, pH 6.0, 4× 5 min. | 1:200 |
Anti-Ki-67 | Thermo Fisher Scientific, Fremont, CA, USA | SP6 rabbit | Boil in citrate buffer, pH 6.0, 3× 5 min. | 1:400 |
Anti-cytokeratin | Sigma-Aldrich, Germany | 8.13 mouse | Boil in citrate buffer, pH 6.0, 2× 5 min. | 1:100 |
Anti-vimentin | Dako, Glostrup, Denmark | V9 mouse | No pre-treatment | 1:400 |
Anti-laminin | Dako, Glostrup, Denmark | Poly rabbit | Protease (Sigma) 1mg/mL PBS, 20 min, room temp. | 1:500 |
Anti-β-catenin | Acris Antibodies, Herford, Germany | 9G2 mouse | Boil in citrate buffer, pH 6.0, 3× 5 min. | 1:100 |