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Figure 4 | BMC Veterinary Research

Figure 4

From: Effects of steroid hormones on differentiated glandular epithelial and stromal cells in a three dimensional cell culture model of the canine endometrium

Figure 4

Immunohistochemical demonstration of oestrogen and progesterone receptorexpression and proliferative activityin 3D cell cultured canine endometrial cells compared to the native tissue. Oestrogen (A) and progesterone (B) receptor expression of glandular epithelial and stromal cells cultured for 48 hr in standard mediumcompared to the native tissue (Coestrogen receptor, Dprogesterone receptor) indicated by immunohistochemical nuclear staining. Proliferative activity in 3D cell-cultured canine glandular epithelial cells (E) (arrows) compared to the in vivo situation (F) of uterine glands and surrounding stromal cells by means of anti-Ki67 immunohistochemical staining. Scale bars A-F, 25µm.

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