| | ctrl | Without FBS |
csf-1rsiRNA | Non-coding siRNA | CSF-1 25 ng/ml | CSF-1 50 ng/ml | CSF-1 100 ng/ml |
|---|
CMT-U27 | 284.0 | 191.0* | 76.00** | 306.5 | 2450*** | 2430*** | 2616*** |
CMT-U309 | 255.0 | 217.5* | 107.0*** | 251.0 | 5114*** | 4080*** | 4100*** |
P114 | 458.5 | 200.0* | 48.50** | 537.0 | 4680*** | 4638*** | 6178*** |
CMT-W1 | 335.0 | 190.0* | 218.5* | 383.5 | 1861** | 2268** | 1780** |
CMT-W2 | 195.5 | 130.8* | 80.50* | 228.5 | 2448*** | 2200*** | 2679*** |
- Mean fluorescence related to CSF-1R expression obtained with FACS Aria II (Becton Dickinson). Mean fluorescence of CSF-1R is significantly decreased after the csf-1r specific siRNA treatment and significantly increased after treatment with CSF-1 at the dose of 25, 50 and 100 ng/ml (no significant differences between these doses have been observed). Treatment of cells with non-coding siRNA did not cause any effect. The experiment has been conducted in three replicates. P<0.05 was regarded as significant and marked as *, whereas p<0.01 and p<0.001 was regarded as highly significant and marked as * and ***, respectively. One-way ANOVA followed by Tukey HSD post-hoc test were applied.
