IgM | IgA | IgG | Commercial ELISA | |
---|---|---|---|---|
Serum ELISAs | ||||
Sample dilution | 1:40 | 1:5 | 1:40 | 1:40 |
Sample volume | 100 μl | 100 μl | 100 μl | 100 μl |
Conjugate dilution | 1:5,000b | 1:1,000c | 1:15,000d | Provided with kit |
Negative control | 100 μl of pooled negative serum diluted 1:40 | 100 μl of pooled negative serum diluted 1:5 | 100 μl of kit negative control | 100 μl of kit negative control |
Positive control | 100 μl of pooled serum from DPI 7 diluted 1:40 | 100 μl of pooled serum from DPI 21 diluted 1:5 | 100 μl of kit positive control | 100 μl of kit positive control |
Oral fluid ELISAs | ||||
Sample dilution | 1:2 | 1:2 | 1:2 | |
Sample volume | 250 μl | 250 μl | 250 μl | |
Conjugate dilution | 1:3,800b | 1:2,000c | 1:2,400d | |
Negative control | 250 μl of reference standard oral fluide DPI 0 diluted 1:2 | 250 μl of reference standard oral fluide DPI 0 diluted 1:2 | 100 μl of kit negative control diluted 1:30 | |
Positive control | 250 μl of reference standard oral fluide DPI 10diluted 1:5 | 250 μl of reference standard oral fluide DPI 91 diluted 1:2 | 100 μl of kit positive control diluted 1:30 |