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Table 4 Summary of porcine reproductive and respiratory syndrome virus (PRRSV) serum and oral fluid antibody enzyme linked-immunosorbent assay (ELISA) condition a

From: Kinetics of the porcine reproductive and respiratory syndrome virus (PRRSV) humoral immune response in swine serum and oral fluids collected from individual boars

 

IgM

IgA

IgG

Commercial ELISA

Serum ELISAs

    

  Sample dilution

1:40

1:5

1:40

1:40

  Sample volume

100 μl

100 μl

100 μl

100 μl

  Conjugate dilution

1:5,000b

1:1,000c

1:15,000d

Provided with kit

  Negative control

100 μl of pooled negative serum diluted 1:40

100 μl of pooled negative serum diluted 1:5

100 μl of kit negative control

100 μl of kit negative control

  Positive control

100 μl of pooled serum from DPI 7 diluted 1:40

100 μl of pooled serum from DPI 21 diluted 1:5

100 μl of kit positive control

100 μl of kit positive control

Oral fluid ELISAs

    

  Sample dilution

1:2

1:2

1:2

 

  Sample volume

250 μl

250 μl

250 μl

 

  Conjugate dilution

1:3,800b

1:2,000c

1:2,400d

 

  Negative control

250 μl of reference standard oral fluide DPI 0 diluted 1:2

250 μl of reference standard oral fluide DPI 0 diluted 1:2

100 μl of kit negative control diluted 1:30

 

  Positive control

250 μl of reference standard oral fluide DPI 10diluted 1:5

250 μl of reference standard oral fluide DPI 91 diluted 1:2

100 μl of kit positive control diluted 1:30

 
  1. a Oral fluid ELISA conditions represent modifications to a commercial PRRSV serum antibody ELISA protocol (IDEXX PRRS X3 Ab Test, IDEXX Laboratories, Inc., Westbrook, ME, USA).
  2. b Anti-pig IgM: HRPO conjugate (A100-100P, Bethyl Laboratories, Montgomery, TX, USA).
  3. c Anti-pig IgA: HRPO conjugate (A100-102P, Bethyl Laboratories, Montgomery, TX, USA).
  4. d Anti-pig IgGFc: HRPO conjugate (A100-104P, Bethyl Laboratories, Montgomery, TX, USA).
  5. e Reference standard oral fluid samples have been previously described [5].