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Table 1 Frequency (%) of Actinobacillus seminis detection by PCR and bacteriology of semen, blood, urine, preputial wash samples from experimentally infected rams during six weeks of infection and from negative control

From: Species-specific multiplex PCR for the diagnosis of Brucella ovis, Actinobacillus seminis, and Histophilus somni infection in rams

Sample

Infected rams

Negative control rams

Agreement (%)c

Kappac

 

Bacteriology

PCR

Bacteriology

PCR

  

Semend

60.0% (42/60) a

90.0% (55/60) b

0.0% (0/27) a

0.0% (0/27) a

79.3

0.59

Bloodd

0.0% (0/60) a

77.1% (53/60) b

0.0% (0/11) a

0.0% (0/11) a

25.4

0.00

Urined

51.4% (36/60) a

48.6% (34/60) a

0.0% (0/8) a

0.0% (0/8) a

67.7

0.35

Preputial washd

38.6% (27/60) a

50.0% (33/60) a

0.0% (0/8) a

0.0% (0/8) a

55.9

0.11

  1. a,b Different letters in the same row indicate statistically significant differences (P < 0.05) according to the Fisher’s exact test.
  2. c Kappa test values considering samples from experimental infection and negative controls.
  3. d These are cumulative results from samples collected weekly throughout the course of infection (up to 42 days post infection).