Table 2 PCR and RT-PCR primers used
Primer name (amplicon length) | Primer sequence (5′ to 3′) |
|---|---|
PCR/ RT-PCR (600) | |
Reverse transcriptase | |
Forward primer | AGCTGGAAACAAGCCACAAT |
Reverse primer | ACAACACTCTTCGGACACCA |
ORF1 (388) | |
Forward primer | CGAGACAAGAAGCAGTTGAAAA |
Reverse primer | TGGGAGCTGGTAAATCTCCT |
ORF2 (249) | |
Forward primer | TCCAGCTTCATCTACATGATCTACC |
Reverse primer | GCATATACTTTGGAAAAAGTGAAAGTC |
ORF3 (276) | |
Forward primer | GGCGACTTTCACTTTTTCCA |
Reverse primer | GCTCGTTTTCGGTTTGGAG |
ORF4 (388) | |
Forward primer | TGAAGAAAGCAGTGCAGGTC |
Reverse primer | GCTTCAGATGCCCCAGTG |
ORF5 (2146) | |
Forward primer | TGGCACACAATCTAAGCATTC |
Reverse primer | CATTCCAGATCCATACCCTGT |
ORF6 (644) | |
Forward primer | TGGAGATCCAATACCTGCTTG |
Reverse primer | TCAATGACCCTTTGTGCTTG |
ORF7 (916) | |
Forward primer | TGAGAAAAGATCAATATTCCATGC |
Reverse primer | TTTGAACTGCATGCTTACCG |
ORF1-7 (4941) | |
Forward primer | CGGCCTGTGCAGACTAATG |
Reverse primer | TTTGAACTGCATGCTTACCG |
ORF4-7 (4114) | |
Forward primer | TGAAGAAAGCAGTGCAGGTC |
Reverse primer | TTTGAACTGCATGCTTACCG |
ASDE | |
Forward primer | CGGCCTGTGCAGACTAATG |
Reverse primer | AGTTAGATTTGCGAAGTCATGC |
5′RACE | |
FirstRt_ASDEF1 | TGCCTGACCCGTTTCCACAACTCT |
NestRt_ASDEF1 | GCTGCCTGGTTTATGGCCTGGATTAG |
FirstRt_ASDEF2 | GCTGCCTGGTTTATGGCCTGGATTAG |
NestRt_ASDEF2 | CTATGTGTGCATTGAAGTCTACCCCTAT |
FirstRt_ASDEF3 | GAAACGGGACGAGACTTCAACACCA |
NestRt_ASDEF3 | TGCAATTGTCGCTCGGAGGTTACTG |
3′RACE | |
FirstLt_ASDER1 | CCGTGGTTGTCTTCCTTGACCTGGA |
NestLt_ASDER1 | TCAGCGTTGTCTGAACCTGGTGTCC |
FirstLt_ASDER2 | TTTGCGGACAATTCCTGGCTCACAC |
NestLt_ASDER2 | CCATGCCTATCCTAAAGGCACAAGCACA |
FirstLt_ASDER3 | CCACTGGCCCTTCCTCCAAATACAA |
NestLt_ASDER3 | CCGCGGTAAGCATGCAGTTCAAAG |