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Table 1 Detection of NLRS in broodstock and their larvae

From: Discovery and partial characterization of a non-LTR retrotransposon that may be associated with abdominal segment deformity disease (ASDD) in the whiteleg shrimp Penaeus (Litopenaeus) vannamei

  

PCR

RT PCR

Four-week broodstock

Broodstock No. 1

+ve

+ve

 

Broodstock No. 2

-ve

-ve

 

Broodstock No. 3

-ve

-ve

 

Broodstock No. 4

-ve

+ve

 

Broodstock No. 5

+ve

-ve

 

Pooled Mysis 3

+ve

+ve

Sixteen-week broodstock

Broodstock No. 1

+ve

+ve

 

Broodstock No. 2

+ve

-ve

 

Broodstock No. 3

+ve

+ve

 

Broodstock No. 4

+ve

+ve

 

Broodstock No. 5

+ve

+ve

 

Pooled Mysis 3

+ve

+ve

  1. Results of polymerase chain reaction (PCR) and reverse transcriptase polymerase chain reaction (RT-PCR) tests specific for the NLRS sequence using nucleic acid extracts from Penaeus vannamei broodstock pleopods and from pooled samples of their larvae (mysis 3). The broodstock specimens were utilized for either four or sixteen weeks in a hatchery.