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Table 2 Real time PCR assay results

From: Ocular and neural distribution of feline herpesvirus-1 during active and latent experimental infection in cats

 

Group 1 (Day 6)

Group 2 (Day 10)

Group 3 (Day 30)

Tissue

RT-PCR

Average FeHV-1 DNA copies/100 cells

RT-PCR

FeHV-1/100 cells

RT-PCR

FeHV-1/100 cells

(#pos/# tested)

(#pos/# tested)

(#pos/# tested)

Cornea

4/4

3915

4/4

1450

1/4

49

Conjunctiva

4/4

6950

4/4

1168

1/4

<1

TG

4/4

106

4/4

262

4/4

93

CG

4/4

65

4/4

409

1/4

1

PTPG

4/4

410

4/4

197

2/4

14

CCG

3/4

1

4/4

4

4/4

3

Uvea

4/4

533

4/4

21

0/4

0

Retina

4/4

2

4/4

4

0/4

0

Optic nerve

4/4

35

4/4

11

1/4

<1

Op chiasm

4/4

<1

4/4

2

0/4

0

Vis cortex

4/4

<1

2/4

<1

0/4

0

Cerebellum

4/4

1

4/4

<1

0/4

0

Brainstem

3/4

3

2/4

<1

0/4

0

Olf bulb

4/4

9

4/4

4

0/4

0

  1. Real-time PCR assay (RT-PCR) results obtained for each tissue type on each day. All control samples were negative and are not listed within this table. TG trigeminal ganglia. CG ciliary ganglia. PTPG pterygopalatine ganglia. CCG cranial cervical ganglia. Op chiasm optic chiasm. Vis cortex visual cortex. Olf bulb olfactory bulb.