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Table 3 Results of the intra-assay and inter-assay testing for clinical specimens

From: Comparison of viremia of type II porcine reproductive and respiratory syndrome virus in naturally infected pigs by zip nucleic acid probe-based real-time PCR

Pig no.

Intra-assay variability

Inter-assay variability

Mean Cq ± SDa

CV%

Mean Cq ± SD

CV%

1

2.09 ± 0.06

2.68

2.13 ± 0.05

2.11

2

2.98 ± 0.14

4.70

3.04 ± 0.14

4.45

3

4.85 ± 0.02

0.43

4.85 ± 0.16

3.39

4

6.01 ± 0.09

1.45

6.07 ± 0.17

2.72

5

2.75 ± 0.13

4.60

2.76 ± 0.10

3.51

  1. aLog10 copy number per microliter of PRRSV.
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BMC Veterinary Research

ISSN: 1746-6148

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