Figure 5

Morphological evidence of replication stress. (A) A nuclear bleb or bud (arrow; bleb/buds are nuclear material still attached but derived from the main nucleus) and a microbody (arrowhead; defined as separate or marginally overlapping with the nucleus, less than one third of its size and with a similar DAPI stain (criteria according to [67])); these structures are evidence of DNA damage, and mitotic stress that can give rise to unstable chromosomal material that is ejected from the cell at the subsequent interphase. DAPI counter-stain (blue). (B) The nuclear bleb and microbody show positive labelling for γH2AX (green) i.e. DNA damage (C) An abnormal, tripolar mitotic figure (TPX2 (red)) with DAPI counter-stain (blue). Scale bars = 10 μm.