Skip to main content
Figure 4 | BMC Veterinary Research

Figure 4

From: Indicators of replicative damage in equine tendon fibroblast monolayers

Figure 4

Association of DNA damage with the replicative fraction. (A) Promyelocytic leukaemia protein (PML) foci (red) juxtaposed with γH2AX puncta (green); as PML bodies are known to locate next to DNA double strand breaks (DSBs), this confirms that many of the γH2AX puncta in our culture system genuinely indicate the presence of DSBs (B) Co-localisation of Rad51 protein (red) and γH2AX (green) as yellow puncta, as further evidence of labelling of DNA breaks. (C) Co-localisation (yellow) of γH2AX puncta with positive labelling for the proliferation marker TPX2 in one mononuclear fibroblast (arrow), indicating a correlation between DNA damage and the replicative fraction. A binucleate cell in the same field contains typical, significant numbers of γH2AX puncta but is arrested (D) The associated DAPI counter-stain (blue) showing all fibroblast nuclei in the field; note the touching, enlarged nuclei in the BN cell (E) The nucleus of a cell that has entered the S/G2 phase of the cell cycle, showing diffuse positive labelling for centromere protein F (green); the same nucleus is the only one in this field that contains γH2AX puncta (in this case, red) i.e. significant DNA damage (F) The associated DAPI counter-stain (blue) showing all of the fibroblast nuclei. Scale bars = 10 μm.

Back to article page