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Figure 3 | BMC Veterinary Research

Figure 3

From: Indicators of replicative damage in equine tendon fibroblast monolayers

Figure 3

Cell cycle arrest in binucleate fibroblasts. These images are taken from the same field (A) Two of these fibroblasts are binucleate (BN), with larger nuclei that are almost touching. DAPI nuclear counter-stain (blue) (B) Nuclei in one of the BN cells show strong diffuse positive labelling for p53 (red), with faint staining of one of the nuclei in the closely adjacent cell; this indicates stabilisation of p53 to facilitate cell cycle arrest and is not seen in the mononuclear cells (C) Both nuclei in both BN cells also show labelling for p21 (green) i.e. the mediator of p53-induced cell cycle arrest (D) Colocalisation of p53 and p21. Scale bars = 10 μm.

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