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Table 5 Inter-assay performance of the TaqMan probe based real time PCR assays

From: Development and validation of TaqMan probe based real time PCR assays for the specific detection of genotype A and B small ruminant lentivirus strains

MVV assay

CAEV assay

Theoretical copy number

Average Ct

Average calculated copy number

CV %

Theoretical copy number

Average Ct

Average calculated copy number

CV %

10 6

19.96

956385

15.76%

10 5

21.39

91881

27.61

10 5

23.34

101936

8.93%

10 4

24.38

12775

26.24

10 4

26.66

11223

8.03%

10 3

27.94

1213

13.19

10 3

30.17

1125

11.44%

10 2

32.07

80

35.18

10 2

34.08

88

29.45%

10

35.21

11

36.03