Figure 4
Capillary-like tube formation on matrigel of primary ECs and EC lines. Phase-contrast images showing angiogenic response of primary (A) and immortalized (C) aortic ECs and primary (B) and immortalized (D) venous ECs, 8 h after plating cells on 24-well plated precoated with matrigel at a density of 105 cells/well. Primary ECs (A-B) mainly exhibited single-cell processes, whereas immortalized ECs (C-D) predominantly reorganized into thicker, multicellular cords. Scale bar, 100 μm.