Skip to main content

Table 2 The primers sequences used for insert DNA amplification of the VP2 and VP3 subunit genes by PCR

From: Expression and characterization of highly antigenic domains of chicken anemia virus viral VP2 and VP3 subunit proteins in a recombinant E. colifor sero-diagnostic applications

Subunit

Primer name

Type

Sequence (5’-3’)

VP2-345N

VP2-NEcoRI

Forward

TGGAATTCATGCACGGGAACGGCGGA

VP2-115C del XhoI

Reverse

TCCTCGAGTGATCGGTCCTCAAGT

VP2-396N

VP2-NEcoRI

Forward

TGGAATTCATGCACGGGAACGGCGGA

VP2-131C del XhoI

Reverse

TCCTCGAGACCCTGTACTCGGAG

VP2-435N

VP2-NEcoRI

Forward

TGGAATTCATGCACGGGAACGGCGGA

VP2-144C del XhoI

Reverse

TCCTCGAGCTGGGAGTAGTGGTAATC

VP2-171C

VP2-170N del EcoRI

Forward

AGGAATTCATGGACGAGCTCGCAGAC

VP2-CXhoI

Reverse

TCCTCGAGCACTATACGTACCGG

VP2-318C

VP2-111N del EcoRI

Forward

TGGAATTCATGGAGGACCGATCAACC

VP2-CXhoI

Reverse

TCCTCGAGCACTATACGTACCGG

VP3-123N

VP3-N EcoRI

Forward

TGGAATTCATGAACGCTCTGCAGGAA

VP3-243C del XhoI

Reverse

TCCTCGAGTCAAGTAATGCCAGCGAT

VP3-246M

VP3-123N del EcoRI

Forward

TGGAATTCATGATTACTCTGTCCCTG

 

VP3-120C del XhoI

Reverse

TCCTCGAGTCATTTCGGCTGATCAGT

VP3-366C

VP3-246N del EcoRI

Forward

TGGAATTCATGCCTCCGTCCAAGAAA

 

VP3-C XhoI

Reverse

TCCTCGAGTTACAGGCGGATGCAACG

  1. Primer names describe the annealing locations of target genes and the restriction enzyme names used in expression plasmid cloning.